Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria
نویسندگان
چکیده
As one of the main non-covalent relations in microbiological-based systems, cell surface hydrophobicity (CSH) can be observed as a relevant parameter for biodegradation capability and suggested bacterial behaviour biofilm formation during bioremediation process. On other hand, role ureolytic bacteria has subsequently led to examination this type different engineering fields. In order optimize operating parameters microbial adhesion hydrocarbons test (MATH) bacteria, Box?Behnken experimental design was conducted five isolated from soils, well reference strain Sporosarcina pasteurii DSM 33. The optimization completed with without essential substrate targeted metabolic reaction, aim compare differences hydrophobicity. A vortex time 2 min, hydrocarbon volume 0.5 mL, phase separation 15 min are recommended MATH all tested bacteria. Although hydrophobic, lower CSH values presence urea were same bacterium, which could explained by interaction organic system, rapid ureolysis process that also occurs application biotechnology systems.
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ژورنال
عنوان ژورنال: Journal of The Serbian Chemical Society
سال: 2021
ISSN: ['1820-7421', '0352-5139']
DOI: https://doi.org/10.2298/jsc200813082s